BARLEY VARIETIES DISCRIMINATION USING MALDI-TOF MASS SPECTROMETRY
نویسندگان
چکیده
منابع مشابه
Fish authentication by MALDI-TOF mass spectrometry.
Recent EU directives and regulations for quality control and authentication of food products have prompted the development of new methods for large-scale tests to ensure the protection of consumers. In view of this, an innovative method based on MALDI-TOF mass spectrometry has been developed and successfully applied to fish authentication. Highly specific mass spectrometric profiles from 25 dif...
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Putative phosphopeptides produced from enzyme hydrolysis of phosvitin were identified and characterised using MALDI-TOF/MS. Phosvitin was heat-pretreated and then hydrolysed using pepsin, thermolysin, and trypsin at their optimal pH and temperature conditions with or without partial dephosphorylation. Pepsin and thermolysin were not effective in producing phosphopeptides, but trypsin hydrolysis...
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Hepcidin has been identified as the principle regulatory hormone essential for iron homeostasis. Quantitative analysis of hepcidin in bodily fluids provides an insight into the pathogenesis of disorders of iron metabolism such as hereditary hemochromatosis and anemia of chronic disease. This study describes the use of solid phase extraction (SPE) as a preparative step followed by matrix assiste...
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Yersinia are Gram-negative, rod-shaped facultative anaerobes, and some of them, Yersinia enterocolitica, Yersinia pseudotuberculosis, and Yersinia pestis, are pathogenic in humans. Rapid and accurate identification of Yersinia strains is essential for appropriate therapeutic management and timely intervention for infection control. In the past decade matrix-assisted laser desorption ionization ...
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Cross-linking can be used to identify spatial relationships between amino acids in proteins or protein complexes. A rapid and sensitive method for identifying the site of protein cross-linking using dithiobis(sulfosuccinimidyl propionate) (DTSSP) is presented and illustrated with experiments using murine cortactin, actin and acyl-CoA thioesterase. A characteristic 66 Da doublet, which arises fr...
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ژورنال
عنوان ژورنال: Journal of Microbiology, Biotechnology and Food Sciences
سال: 2019
ISSN: 1338-5178
DOI: 10.15414/jmbfs.2019.8.6.1347-1351